##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	mutant_R1
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12480
Sequences flagged as poor quality	0
Sequence length	150
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.42051282051282	30.0	30.0	30.0	28.0	32.0
2	29.645432692307693	30.0	30.0	30.0	28.0	32.0
3	29.77411858974359	30.0	30.0	30.0	28.0	32.0
4	29.92900641025641	30.0	30.0	30.0	30.0	32.0
5	29.828846153846154	30.0	30.0	30.0	30.0	32.0
6	31.823317307692307	33.0	32.0	33.0	30.0	33.0
7	31.75272435897436	33.0	32.0	33.0	30.0	33.0
8	31.856410256410257	33.0	32.0	33.0	30.0	33.0
9	33.788621794871794	35.0	33.0	35.0	31.0	35.0
10-14	33.86544871794872	35.0	33.4	35.0	31.0	35.0
15-19	35.49782051282051	36.2	36.2	36.8	33.0	36.8
20-24	37.140032051282056	37.8	37.0	38.8	34.0	38.8
25-29	37.9455608974359	39.0	37.0	40.0	34.0	40.0
30-34	37.9998076923077	39.0	37.8	40.0	34.8	40.0
35-39	38.02495192307693	39.0	38.0	40.0	35.0	40.0
40-44	37.99107371794872	39.0	38.0	40.0	35.0	40.0
45-49	37.888141025641026	39.0	38.0	40.0	35.0	40.0
50-54	37.78786858974359	39.0	37.2	40.0	35.0	40.0
55-59	37.68358974358974	39.0	37.0	40.0	34.8	40.0
60-64	37.64485576923077	39.0	37.0	40.0	34.2	40.0
65-69	37.6509935897436	39.0	37.0	40.0	34.4	40.0
70-74	37.777243589743584	39.0	37.2	40.0	35.0	40.0
75-79	37.67190705128205	39.0	37.0	40.0	35.0	40.0
80-84	37.588830128205124	39.0	37.0	40.0	34.2	40.0
85-89	37.53416666666667	39.0	37.0	40.0	34.2	40.0
90-94	37.399583333333325	39.0	37.0	39.6	34.2	40.0
95-99	37.27165064102564	39.0	37.0	39.0	34.0	40.0
100-104	37.05940705128206	39.0	37.0	39.0	34.0	40.0
105-109	36.874551282051286	39.0	37.0	39.0	34.0	39.0
110-114	36.593301282051286	38.2	36.8	39.0	33.8	39.0
115-119	36.316298076923076	38.0	36.0	39.0	33.0	39.0
120-124	36.01107371794872	38.0	36.0	39.0	32.4	39.0
125-129	35.75059294871795	37.2	36.0	38.0	31.4	39.0
130-134	35.43491987179487	37.0	36.0	38.0	30.8	38.8
135-139	35.16259615384616	37.0	35.8	38.0	30.2	38.0
140-144	35.00673076923077	37.0	35.6	38.0	30.0	38.0
145-149	35.21700320512821	37.0	36.0	38.0	30.0	38.0
150	35.173878205128204	37.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
34	8.0
35	1299.0
36	10254.0
37	919.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.403846153846153	33.14102564102564	32.43589743589744	17.01923076923077
2	16.41025641025641	33.62980769230769	33.48557692307692	16.474358974358974
3	16.786858974358974	34.07852564102564	32.70032051282051	16.434294871794872
4	16.690705128205128	33.381410256410255	33.10096153846154	16.826923076923077
5	16.193910256410255	33.48557692307692	34.118589743589745	16.201923076923077
6	17.251602564102562	32.93269230769231	32.98076923076923	16.834935897435898
7	17.01121794871795	33.03685897435897	32.96474358974359	16.98717948717949
8	16.105769230769234	33.50160256410256	33.85416666666667	16.538461538461537
9	17.32371794871795	32.96474358974359	33.0849358974359	16.626602564102562
10-14	16.503205128205128	33.55769230769231	32.97115384615385	16.96794871794872
15-19	16.743589743589745	32.91346153846153	33.46794871794872	16.875
20-24	16.996794871794872	33.131410256410255	33.06570512820513	16.806089743589745
25-29	16.84294871794872	32.9599358974359	32.96794871794872	17.229166666666668
30-34	16.849358974358974	33.41346153846153	33.05769230769231	16.67948717948718
35-39	16.701923076923077	33.429487179487175	33.23717948717949	16.631410256410255
40-44	17.041666666666664	32.82211538461539	33.419871794871796	16.716346153846153
45-49	16.84294871794872	33.20192307692308	32.99519230769231	16.959935897435898
50-54	16.564102564102566	33.58333333333333	33.16025641025641	16.692307692307693
55-59	16.73717948717949	33.293269230769226	33.06410256410256	16.90544871794872
60-64	16.897435897435898	33.16025641025641	32.97275641025641	16.96955128205128
65-69	16.703525641025642	33.55288461538461	32.94871794871795	16.794871794871792
70-74	16.60096153846154	33.144230769230774	33.39102564102564	16.86378205128205
75-79	16.78525641025641	33.17788461538462	33.3525641025641	16.684294871794872
80-84	16.649038461538463	33.08653846153846	33.07211538461539	17.192307692307693
85-89	16.897435897435898	33.01442307692308	33.27564102564102	16.8125
90-94	16.78044871794872	33.07852564102564	33.28846153846154	16.852564102564102
95-99	16.782051282051285	33.270833333333336	33.043269230769226	16.903846153846153
100-104	17.072115384615387	33.32852564102564	32.929487179487175	16.669871794871796
105-109	16.753205128205128	33.17307692307692	33.15705128205128	16.916666666666664
110-114	16.915064102564102	33.03525641025641	33.32051282051282	16.729166666666668
115-119	16.73878205128205	33.17628205128205	33.21314102564102	16.871794871794872
120-124	17.104166666666668	33.116987179487175	33.21634615384615	16.5625
125-129	17.100961538461537	33.01602564102564	33.06730769230769	16.815705128205128
130-134	16.82371794871795	33.21314102564102	33.20673076923077	16.756410256410255
135-139	16.751602564102562	33.00160256410257	33.37019230769231	16.876602564102562
140-144	16.875	33.30929487179487	33.11057692307692	16.705128205128204
145-149	16.581730769230766	33.416666666666664	33.1875	16.814102564102566
150	17.147435897435898	32.82051282051282	33.46153846153846	16.57051282051282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.5
14	5.0
15	10.0
16	17.5
17	25.0
18	29.5
19	38.5
20	59.5
21	83.0
22	115.0
23	138.0
24	187.5
25	253.0
26	312.5
27	374.0
28	439.5
29	552.5
30	642.0
31	722.0
32	768.5
33	811.0
34	873.5
35	858.5
36	816.5
37	772.0
38	700.0
39	606.5
40	519.5
41	448.5
42	364.0
43	271.0
44	205.0
45	154.0
46	107.0
47	71.5
48	45.5
49	30.0
50	19.0
51	9.0
52	6.0
53	7.0
54	5.5
55	2.5
56	1.0
57	1.0
58	0.5
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	12480.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.19711538461539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.1640146878825	96.39423076923077
2	1.8359853121175032	3.6057692307692304
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE